Statistical Methods for Spot Detection with Macroarray Data.
Yi Xie, Adele Cutler, Bart Weimer, Andrejus Parfionovas
Abstract :
We describe a statistical method for detecting spots and extracting intensities for an oligonucleotide macroarray. The method is freely available, implemented as an ImageJ plugin. Special capabilities include a fast automatic grid finder, detection of very weak spots, and handling saturation of strong spots due to limitations in the dynamic range of the detection device. The method is validated on dilution arrays and illustrated with experimental data.
Software :
Software is provided in the form of an ImageJ plugin. The plugin requires the the java matrix package JAMA and the optimization package Minpack_f77 .